Another paper on the purification of anti-HSA IgG1 MAb using CIM-IDA disks immobilized with four different metal ions. This time, the authors report superior purification with Zn (II) and Co (II) ions, with Zn (II)) being supposedly far better.
Again, similar problems as before: images with brightness and contrast adjusted to a point where the marker (especially in the "best" Zn (II) case) itself has lost some bands. In fact, the only ion with which they get one band is with Co (II) but its recovery does not appear to be very good.
Ironically in this paper, the authors make a statement (shown below) about how acidic pH is bad for their antibody. Given that the senior authors appear to have published many papers using pH 4 or pH 5.5 elution or equilibriation conditions (please see other posts in this blog), why this sudden discovery and what about those earlier antibodies and reports?
Poonam Rajak et al. 2012 Ambur P Dhivya et al. 2010
Fairly clean blot compared to other blots from this group with only slight degradation evident in the IgG1 MAb lane.
Rajak P, Vijayalakshmi MA, & Jayaprakash NS (2012). Purification of monoclonal antibodies, IgG1, from cell culture supernatant by use of metal chelate convective interaction media monolithic columns Biomedical Chromatography, 1488-1493 DOI: 10.1002/bmc.2721
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